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rfp filter set at 63 he  (Carl Zeiss)
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Carl Zeiss rfp filter set at 63 he
Generation of a transgenic medaka strain Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . (A) Schematic illustration of a non-homologous end joining (NHEJ)-mediated knock-in strategy. CoChR, a blue light-sensitive channelrhodopsin from Chloromonas oogama , and mScarlet, a red fluorescent protein <t>(RFP),</t> were fused in-frame with a 2A peptide sequence derived from porcine teschovirus (P2A). The expression cassette was driven under the basal promoter derived from the Xenopus β-globin gene (Xhbb). Yellow boxes indicate the sgRNA target region. (B-G) Expression patterns of the <t>RFP</t> <t>fluorescence</t> at the larval stage of Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . Images with a GFP (B, E) or RFP (C, D) filter set show the autofluorescence or RFP signals, respectively. In the merged images (D, G), white arrows or blue arrowheads indicate RFP signals in ganglions mentioned below or autofluorescence signals, respectively. Both the lateral (B-D) and dorsal (E-G) views are shown. RGCs, retinal ganglion cells; aRGCs, axon of RGCs; V, trigeminal nerve; Va, anterior V; Vp, posterior V; VII, facial nerve; X, vagus nerve; PG, pineal gland; SMN, spinal motor neurons. Scale bars, 500 μm.
Rfp Filter Set At 63 He, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/filter+set+(he)+rfp/15+filter+set/bio_rxiv__2023__04__05__535638-41-19-7
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rfp filter set at 63 he - by Bioz Stars, 2026-10
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filter set (he) rfp/ gfp/dapi  (Carl Zeiss)
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Carl Zeiss filter set (he) rfp/ gfp/dapi
Generation of a transgenic medaka strain Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . (A) Schematic illustration of a non-homologous end joining (NHEJ)-mediated knock-in strategy. CoChR, a blue light-sensitive channelrhodopsin from Chloromonas oogama , and mScarlet, a red fluorescent protein <t>(RFP),</t> were fused in-frame with a 2A peptide sequence derived from porcine teschovirus (P2A). The expression cassette was driven under the basal promoter derived from the Xenopus β-globin gene (Xhbb). Yellow boxes indicate the sgRNA target region. (B-G) Expression patterns of the <t>RFP</t> <t>fluorescence</t> at the larval stage of Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . Images with a GFP (B, E) or RFP (C, D) filter set show the autofluorescence or RFP signals, respectively. In the merged images (D, G), white arrows or blue arrowheads indicate RFP signals in ganglions mentioned below or autofluorescence signals, respectively. Both the lateral (B-D) and dorsal (E-G) views are shown. RGCs, retinal ganglion cells; aRGCs, axon of RGCs; V, trigeminal nerve; Va, anterior V; Vp, posterior V; VII, facial nerve; X, vagus nerve; PG, pineal gland; SMN, spinal motor neurons. Scale bars, 500 μm.
Filter Set (He) Rfp/ Gfp/Dapi, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/filter+set+(he)+rfp/microscope+zeiss/pm35046576-551-30-35
Average 90 stars, based on 1 article reviews
filter set (he) rfp/ gfp/dapi - by Bioz Stars, 2026-10
90/100 stars
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Generation of a transgenic medaka strain Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . (A) Schematic illustration of a non-homologous end joining (NHEJ)-mediated knock-in strategy. CoChR, a blue light-sensitive channelrhodopsin from Chloromonas oogama , and mScarlet, a red fluorescent protein (RFP), were fused in-frame with a 2A peptide sequence derived from porcine teschovirus (P2A). The expression cassette was driven under the basal promoter derived from the Xenopus β-globin gene (Xhbb). Yellow boxes indicate the sgRNA target region. (B-G) Expression patterns of the RFP fluorescence at the larval stage of Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . Images with a GFP (B, E) or RFP (C, D) filter set show the autofluorescence or RFP signals, respectively. In the merged images (D, G), white arrows or blue arrowheads indicate RFP signals in ganglions mentioned below or autofluorescence signals, respectively. Both the lateral (B-D) and dorsal (E-G) views are shown. RGCs, retinal ganglion cells; aRGCs, axon of RGCs; V, trigeminal nerve; Va, anterior V; Vp, posterior V; VII, facial nerve; X, vagus nerve; PG, pineal gland; SMN, spinal motor neurons. Scale bars, 500 μm.

Journal: bioRxiv

Article Title: Optogenetic control of medaka behavior with channelrhodopsin

doi: 10.1101/2023.04.05.535638

Figure Lengend Snippet: Generation of a transgenic medaka strain Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . (A) Schematic illustration of a non-homologous end joining (NHEJ)-mediated knock-in strategy. CoChR, a blue light-sensitive channelrhodopsin from Chloromonas oogama , and mScarlet, a red fluorescent protein (RFP), were fused in-frame with a 2A peptide sequence derived from porcine teschovirus (P2A). The expression cassette was driven under the basal promoter derived from the Xenopus β-globin gene (Xhbb). Yellow boxes indicate the sgRNA target region. (B-G) Expression patterns of the RFP fluorescence at the larval stage of Tg(isl1-Xhbb:CoChR-P2A-mScarlet) . Images with a GFP (B, E) or RFP (C, D) filter set show the autofluorescence or RFP signals, respectively. In the merged images (D, G), white arrows or blue arrowheads indicate RFP signals in ganglions mentioned below or autofluorescence signals, respectively. Both the lateral (B-D) and dorsal (E-G) views are shown. RGCs, retinal ganglion cells; aRGCs, axon of RGCs; V, trigeminal nerve; Va, anterior V; Vp, posterior V; VII, facial nerve; X, vagus nerve; PG, pineal gland; SMN, spinal motor neurons. Scale bars, 500 μm.

Article Snippet: Imaging was performed using an Axio Zoom.V16 (Zeiss) with a green fluorescence protein (GFP) filter set at 38 HE, RFP filter set at 63 HE, the Apotome 3 system, and a monochrome Axiocam 705 mono camera.

Techniques: Transgenic Assay, Non-Homologous End Joining, Knock-In, Sequencing, Derivative Assay, Expressing, Fluorescence